OB3 Allophycocyanin (APC) is isolated from Spirulina sp., a blue-green alga. APC is fluorescent, with an extremely high absorptivity and a high quantum efficiency. Without altering its spectral characteristics, it is a protein which can be easily linked to antibodies and other proteins by conventional protein cross-linking techniques.
MOQ: 10 mg.
Bulk Price Promotion: please contact us for more details.
From
Cyanophyta
Molecular Weight
105 kDa
Subunit Structure
(αβ)3
Absorption Maximum
651 nm± 2nm
Emission Maximum
660 nm± 2nm
Extinction Coefficient
7.0 x 105 cm-1 M-1
Protein Concentration
≧15 mg/ml
Purity
A651/A280 ≥ 5.0
A651/A620 ≥ 1.5
A651/A620 ≥ 1.5
Stability
Supplied in 100 ml sodium phosphate buffer pH7.0 with 60% ammonium sulfate, 1mM EDTA, and 1mM sodium azide
Shelf life
2 years under proper storage conditions.
Storage
Store at 4°C, away from light. Do not freeze.
Origin
Taiwan
Preparation Before Use
APC is stored in ammonium sulfate solution. Therefore, APC needs to be re-suspended until no visible precipitates. Firstly, it must be extensively dialyzed prior to use or column to remove any remaining ammonium sulfate. Use PBS or your conjugation buffer (1 ml of APC to 1 liter of buffer) to dialyze at least two changes of buffer.
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One BioTech Inc.
No. 168, Chaoma Road, Taichung, Taiwan.
+886-931529910
services@one-biotech.com
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