OB2

Cross-linked Allophycocyanin

OB2 Cross-linked Allophycocyanin (XL-APC) is the brightest and most stable form of APC available. The αβ subunits of the APC have been crosslinked in order to provide increased structural integrity in the presence of chaotropic salts.

MOQ: 10 mg.
Bulk Price Promotion: please contact us for more details.

From
Cyanophyta
Molecular Weight
105 kDa
Subunit Structure
(αβ)3
Absorption Maximum
650 nm± 2nm
Emission Maximum
660 nm± 2nm
Extinction Coefficient
7.0 x 105 cm-1 M-1
Protein Concentration
≧15 mg/ml
Purity
A650/A280 ≥ 5.0
A650/A620 ≥ 1.5
Cross-link ratio ≥ 1.2
Stability
Supplied in 100 mM sodium phosphate buffer pH7.0 with 60% ammonium sulfate, 1mM EDTA, and 1mM sodium azide
Shelf life
2 years under proper storage conditions.
Storage
Store at 4°C, away from light. Do not freeze.
Origin
Taiwan
Preparation Before Use
XL-APC is stored in ammonium sulfate solution. Therefore, XL-APC needs to be re-suspended until no visible precipitates. Firstly, it must be extensively dialyzed prior to use or column to remove any remaining ammonium sulfate. Use PBS or your conjugation buffer (1 ml of XL-APC to 1 liter of buffer) to dialyze at least two changes of buffer.

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